Traditional herbal formula Jakyakgamcho-tang (Paeonia lactiflora and Glycyrrhiza uralensis) impairs inflammatory chemokine production by inhibiting activation of STAT1 and NF-κB in HaCaT cells
Soo-Jin Jeong, Hye-Sun Lim, Chang-Seob Seo, Jung-Hoon Kim, Seong-Eun Jin, Sae-Rom Yoo, Hyeun-Kyoo Shin,
A traditional herbal formula Jakyakgamcho-tang (JYGCT; Paeonia lactiflora andGlycyrrhiza uralensis) has been used for treatment of backache, muscle pain, acute abdominal pain, neuralgia, bronchial asthma, and painful peripheral neuropathy in Oriental medicine. We report on our experiments using the HaCaT human keratinocyte cell line showing that a traditional herbal formula JYGCT has inhibitory effects on inflammatory responses in skin.
Stimulation with tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) caused a significant increase in the production of the following chemokines: thymus- and activation-regulated chemokine (TARC)/CCL17; macrophage-derived chemokine (MDC)/CCL22; regulated on activation, normal T-cell expressed and secreted (RANTES)/CCL5; and interleukin-8 (IL-8) in HaCaT cells. By contrast, treatment with
Oxypaeoniflorin, isoliquiritin, ononin, and benzoylpaeoniflorin were obtained from Biopurify Phytochemicals (Chengdu, China).
