Effect of green tea extract and epigallocatechin-3-gallate potency on lipid profile and coronary artery morphology of dyslipidemic rats

Wahyu Widowati, Hana Ratnawati, Roro Wahyudianingsih, Yelliantty Yelliantty, Maesaroh Maesaroh, Dwiyati Pujimulyani, Tjandrawati Mozef.

Abstract

Objective: Dyslipidemia is a major risk factor in atherosclerosis and cardiovascular disease. Green tea and catechins have anti-dyslipidemic potency. This research was carried out to evaluate the effects of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on lipid profile, antioxidant activities and histopathology of coronary artery in dyslipidemic rats. 
Methods: Six weeks old male Sprague-Dawley rats were fed high cholesterol diet for 2 month. Three groups were treated with green tea extract (450, 300 and 150 mg/kg body weight/daily), another 3 groups were treated with EGCG (15, 10 and 5 mg/kg BW/daily), and a final group remained as untreated positive control. The treatment duration was 21 and 42 days. Lipid profile including total cholesterol (TC), triglyceride (TG), low and high density lipoprotein (LDL-HDL) levels, superoxide dismutase (SOD) activity, malondialdehyde (MDA) and coronary artery histophatology were evaluated. Data was analyzed using ANOVA and Duncan post hoc test. 

EGCG-treated groups (n = 5 for all) (Chengdu Biopurify Phytochemicals, Sichuan, PR China