Abstract
BACKGROUND
Purple corn (Zea mays L.) is one of the main economic crops in China and has been used in the treatment of cystitis, urinary infections and obesity. However, purple corncobs, the by‐product remaining after processing and having an intense purple‐black color, are normally disposed of as waste or used as animal feed. Therefore, to further expand the medicinal value of purple corncob, its content was analyzed and, after purification, the effect and mechanism of purified purple corncob anthocyanins (PPCCA) on CCl4‐induced chronic liver injury in mice were investigated.
RESULTS
It was observed that the total anthocyanin content (TAC) from PPCCA (317.51 ± 9.30 mg cyanidin 3‐O‐glucoside (C‐3‐G) g−1 dry weight) was significantly higher than that from the purified purple corn seed anthocyanin (266.73 ± 3.67 mg C‐3‐G g−1 dry weight), of which C‐3‐G accounted for 90.6% and 90.4% of the TAC, respectively. Furthermore, compared with the CCl4 group, PPCCA treatment significantly reduced liver index, serum total bilirubin, alanine transaminase, aspartate transaminase and liver malondialdehyde levels, but increased liver superoxide dismutase activity. The pathological changes were also improved, such as more regular arrangement of hepatocytes, less swelling, and fewer vacuoles and apoptotic cells. Additionally, mechanistic studies showed that PPCCA downregulated the expression of Caspase‐3, Bax and cytochrome P450 2E1 proteins in the liver and upregulated the expression of Bcl‐2.
CONCLUSION
These results demonstrated that PPCCA could ameliorate CCl4‐induced chronic liver injury by regulating oxidative stress and hepatocyte apoptosis pathways. © 2021 Society of Chemical Industry
… collected in Hei Longjiang province, China. Standard cyanidin-3-O-glucoside (C-3-G) was purchased from Biopurify Phytochemicals Ltd., Sichuan, China. Diaion HP2 MGL macroporous resin was purchased from Mitsubishi, Japan. Alanine transaminase …
