Abstract Purpose: Recently, the demand for natural extracts and naturally derived ingredients from core raw materials has increased in the cosmetic industry, but their manufacturing and quality control standards are not sufficiently established. This study aims to standardize the compositions of marker compounds from natural cosmetics using ethanol extracts of Chrysanthemum indicum (CI) and Cymbopogon cirtratus (CC). The method is then validated. Methods: In liquid chromatography (LC)/mass spectrometry, astragalin and p-coumaric acid were determined as the marker compounds for quantitative analysis. The performances (linearity, accuracy, and precision) of these two marker components were simultaneously analyzed in a mixture of both natural products using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Results: The marker compounds of CI and CC in the extracts were determined as astragalin and p-coumaric acid, respectively. The detected marker compounds from the CI and CC showed significant linearity (R2 ≥0.9999). The astragalin and p-coumaric acid markers achieved limits of detection of 1.20 and 0.07 ng/mL, respectively, and limits of quantification of 3.59 and 0.22 ng/mL, respectively. The marker concentrations were 0.24 μg/mL in CI and 1.55 μg/mL in CC. Conclusion: This study suggests that the developed method may be an important basic data for the manufacturing and quality control of natural cosmetic.
… 시료로 사용하였다. 추 출 수율은 약 17% 이었다. 2. 시약 표준품 astragaline, p-coumaric acid는 HPLC grade (순도 98% 이상)로 Chengdu Biopurify Phytochemicals (Chengdu, China)에서 구입하여 사용하였다. Acetonitrile, methanol …
