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沈帅 
文静
贺丹丹
文献信息
Rapid identification and determination of pyrrolizidine alkaloids in herbal and food samples via direct analysis in real-time mass spectrometry
Abstract
Pyrrolizidine alkaloids (PAs) are naturally occurring plant toxins associated with severe liver damage if excessive ingestion. Herein, a novel analytical strategy on utilizing direct analysis in real-time mass spectrometry (DART-MS) was developed, and applied in analysis of six representative PAs. The calibration curves in the range of 10–1000 ng·mL−1 were established, and relative standard deviations (RSDs) were less than 10%. The limits of detection (LODs) and limits of quantitation (LOQs) were 0.55–0.85 ng·mL−1 and 1.83–2.82 ng·mL−1, respectively. The feasibility of method was indicated by analysing real samples including Gynura japonica, drug tablets, granules, and fresh cow’s milk. Moreover, the results of DART-MS were in good agreement with those observed by high performance liquid chromatography mass spectrometry (HPLC-MS), but consumed less time without chromatographic separation. This research provides a facile fashion for safety assessment of herbal and food products containing PAs and presents promising applications in food, pharmaceutical and clinical analysis.
… purification unless otherwise specified. Senecionine (SEN), seneciphylline (SENP), senkirkine (SENK) and retrorsine (RETRO) were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). Adonifoline (ADO) and …
