Quantification of phenolic acids and their methylates, glucuronides, sulfates and lactones metabolites in human plasma by LC–MS/MS after oral ingestion of soluble coffee

Cynthia Marmet, , Lucas Actis-Goretta, Mathieu Renouf, Francesca Giuffrida

Abstract

Chlorogenic acids and derivatives like phenolic acids are potentially bioactive phenolics, which are commonly found in many foods. Once absorbed, chlorogenic and phenolic acids are highly metabolized by the intestine and the liver, producing glucuronidated and/or sulphated compounds. These metabolites were analyzed in human plasma using a validated liquid chromatography-electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) method. After protein precipitation, phenolic acids and their metabolites were extracted by using ethanol and chromatographic separation was achieved by reversed-phase using an Acquity UPLC BEH C18 column

3-caffeoylquinic acid (3CQA), 4-caffeoylquinic acid (4CQA) were obtained from Chengdu Biopurify Phytochemicals Ltd (Chengdu, Sichuan, China). ...