在线咨询
联系电话:
销售：
400-829-7929（7*24小时）
028-
82633860
028-
82633397
028-82633165
技术服务和产品定制：
028-82633987
在线服务:
沈帅 
文静
贺丹丹
文献信息
Antioxidant Activity Evaluation of Dietary Flavonoid Hyperoside Using Saccharomyces Cerevisiae as a Model
Abstract: Oxidative stress leads to various diseases, including diabetes, cardiovascular diseases, neurodegenerative diseases, and even cancer. The dietary flavonol glycoside, hyperoside (quercetin-3-O-galactoside), exerts health benefits by preventing oxidative damage. To further understand its antioxidative defence mechanisms, we systemically investigated the regulation of hyperoside on oxidative damage induced by hydrogen peroxide, carbon tetrachloride, and cadmium in Saccharomyces cerevisiae. Hyperoside significantly increased cell viability, decreased lipid peroxidation, and lowered intracellular reactive oxygen species (ROS) levels in the wild-type strain (WT) and mutants gtt1∆ and gtt2∆. However, the strain with ctt1∆ showed variable cell viability and intracellular ROS-scavenging ability in response to the hyperoside treatment upon the stimulation of H2O2 and CCl4. In addition, hyperoside did not confer viability tolerance or intercellular ROS in CdSO4-induced stress to strains of sod1∆ and gsh1∆. The results suggest that the antioxidative reactions of hyperoside in S. cerevisiae depend on the intercellular ROS detoxification system.
… (Tianjin, China). Dimethyl sulfoxide (DMSO), yeast extract, and peptone were purchased from Oxoid Company (Basingstoke, UK). Glucose was purchased from Amresco (Solon, OH, USA). Hyperoside was purchased from Biopurify Technology Development Co., Ltd …
