Abstract

The anti-tumor effects of catechins and gallic acid (GA) in-vitro was investigated in this paper. Fluo-3AM, Calcium-AM (Ca-AM), 2′, 7′ -dichlorofluorescein-diacetate(DCFH-DA), 4′, 6-diamidino-2- phenylindole (DAPI) and Ca-AM plus colbat were used to characterize intracellular calcium, labile iron pool (LIP), reactive oxygen species (ROS), nuclei morphology and mitochondrial permeability transition pore (mPTP) opening, respectively. High performance liquid chromatography (HPLC) was used to quantitate catechins and GA in the cultural medium. The results indicated that each of them showed dose response inhibition of cell growth, provoking nuclei condensation, intracellular calcium elevation, mPTP opening, LIP reduction, and cytochrome c (Cyt-C) to release into cytosol. The caspase

 (?)-epigallocatechin gallate (EGCG),(?)epicatechin gallate (ECG), (?)epigallocate- chin (EGC), and Gallic acid (GA) (Chengdu Biopurify Phytochemicals Co., Ltd, China