Luteolin decreases the attachment, invasion and cytotoxicity of UPEC in bladder epithelial cells and inhibits UPEC biofilm formation

Xiao-fei Shen, Lai-bin Ren, Yan Teng, Shuang Zheng, Xiao-long Yang,

Xiao-juan Guo, Xin-yuan Wang, Kai-hui Sha, Na Li, Guang-ya Xu, 

Han-wen Tian, Xiao-ying Wang, Xiao-kang Liu, Jingyu Li , Ning Huang , 

Abstract

Urinary tract infection (UTI), primarily caused by uropathogenic Escherichia coli (UPEC), is one of the most common infectious diseases worldwide. Emerging antibiotic resistance requires novel treatment strategies. Luteolin, a dietary polyphenolic flavonoid, has been confirmed as a potential antimicrobial agent. Here, we evaluated the sub-MICs of luteolin for potential properties to modulate the UPEC infection. We found that luteolin significantly decreased the attachment and invasion of UPEC J96 or CFT073 in human bladder epithelial cell lines T24. Meanwhile, obvious decreased expression of type 1 fimbriae adhesin fimH gene, lower bacterial surface hydrophobicity and

Luteolin (98% purity) was purchased from Chengdu Biopurify Phytochemicals Co., Ltd. (Chengdu, China).