Abstract
Ginsenoside Rh2, a rare protopanaxadiol (PPD)-type triterpene saponin isolated from Panax ginseng, exhibits notable anticancer and immune-system-enhancing activities. Glycosylation catalyzed by uridine diphosphate-dependent glucosyltransferase (UGT) is the final biosynthetic step of ginsenoside Rh2. In this study, UGT73C5 isolated from Arabidopsis thalianawas demonstrated to selectively transfer a glucosyl moiety to the C3 hydroxyl group of PPD to synthesize ginsenoside Rh2. UGT73C5 was coupled with sucrose synthase (SuSy) from A. thaliana to regenerate costly uridine diphosphate glucose (UDPG) from cheap sucrose and catalytic amounts of uridine diphosphate (UDP). The UGT73C5/SuSy ratio, temperature, pH, cofactor UDP, and PPD concentrations for UGT73C5–SuSy coupled reactions were optimized. Through the stepwise addition of PPD, the maximal ginsenoside Rh2 production was 3.2 mg mL−1, which was the highest yield reported to date. These promising results provided an efficient and cost-effective approach to semisynthesize the highly valuable ginsenoside Rh2.
… acceptor, respectively. 2. Materials and methods. 2.1. Chemicals and reagents. PPD and PPD-type ginsenosides (Rh2, CK, F2, and Rg3) were provided by Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). UDP, UDPG …
Biocatalytic synthesis of ginsenoside Rh2 using Arabidopsis thaliana glucosyltransferase-catalyzed coupled reactions
期刊名：Journal of Biotechnology
文献编号：
文献地址： https://www.sciencedirect.com/science/article/pii/S0168165620300031
发表日期：10 February 2020
文献编号：
文献地址： https://www.sciencedirect.com/science/article/pii/S0168165620300031
发表日期：10 February 2020
