Abstract
Simultaneous analysis of 20 marker components (gallic acid, cimifugin, geniposide, paeoniflorin, ferulic acid, nodakenin, narirutin, naringin, neohesperidin, arctiin, baicalin, oxypeucedanin hydrate, wogonoside, baicalein, arctigenin, glycyrrhizin, wogonin, pulegone, decursin, and decursinol angelate) for quality assessment of the traditional herbal formula, Hyeonggaeyeongyo-tang (HYT) was carried out by using high-performance liquid chromatography (HPLC) with photodiode array detection (PDA) and liquid chromatography–mass spectrometry with tandem mass spectrometry (LC–MS/MS). The coefficient of determination showed excellent linearity of more than 0.9999 for all analytes. The recovery of 20 marker components was 93.92 to 102.66% with relative standard deviation (RSD) < 3.00% and RSD value of precision was ≤ 3.44%. The amounts of 20 marker components using HPLC–PDA and LC–MS/MS were determined to be 0.18–14.60 and 0.01–1.76 mg/freeze-dried g, respectively.
… (Wuhan, China); narirutin (99.5%), glycyrrhizin (99.1%), and pulegone (99.3%) were purchased from Biopurify Phytochemicals (Chengdu, China); geniposide (≥98.0%) and baicalin (98.0%) were purchased from Wako Chemicals …
Quality assessment of traditional herbal formula, Hyeonggaeyeongyo-tang through simultaneous determination of twenty marker components by HPLC–PDA and LC–MS/MS
期刊名：Saudi Pharmaceutical Journal
文献编号：
文献地址： https://www.sciencedirect.com/science/article/pii/S1319016420300311
发表日期：17 February 2020
文献编号：
文献地址： https://www.sciencedirect.com/science/article/pii/S1319016420300311
发表日期：17 February 2020
