Abstract

The aim of this study was to confirm pharmacokinetic screening of multiple components in healthy Korean subjects after oral administration of Samso‐eum and perform quantitation of active components in the human plasma. Thirteen potential bioactive components (puerarin, daidzin, nodakenin, ginsenoside Rb1, 18β‐glycyrrhetinic acid, 6‐shogaol, naringin, glycyrrhizin, hesperidin, platycodin D, naringenin, hesperetin, and 6‐gingerol) based on literature were screened. The results showed that three analytes (daidzin, naringenin and hesperetin) were detected in trace amounts. In addition, puerarin and 18β‐ glycyrrhetinic acid were detected in human plasma after the oral administration of Samso‐eum. In this study, a liquid chromatography–electrospray ionization‐tandem mass spectrometry (LC–ESI‐MS/MS) method was validated for the simultaneous determination of puerarin and 18β‐glycyrrhetinic acid in human plasma. This was the first study to evaluate pharmacokinetics of puerarin and 18β‐glycyrrhetinic acid after usual oral dose of Samso‐eum (30 g containing 102.48 mg puerarin, 48.18 mg glycyrrhizin) in human subjects.

… Compound PRR, daidzin, 18β-GTA, naringin, hesperidin, naringenin,
hesperetin (Sigma, St Louis, MO, USA), 6-shogaol (Biopurify, Chengdu, China),
platycodin D (Chemfaces, Wuhan, China >98 %), nodakenin, ginsenoside …