Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD–ESI-MSnand screening for their antioxidant activity

Vítor Spínola, Joana Pinto, Paula C. Castilho, 

Abstract

Five fruits species commonly cultivated and consumed in Madeira Island (Portugal) were investigated for their phenolic profile by means of reversed phase high-performance liquid chromatography coupled to diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD–ESI/MSn) and antioxidant potential. A large number of compounds were characterised, flavonoids and phenolic acids being the major components found in target samples, 39 compounds (flavonoids, phenolic acids, terpenoids, cyanogenic glycosides and organic acids) were identified in cherimoyas, lemons, papayas, passion-fruits and strawberries for the first time.

gallic acid (GA), quercetin (QC), and potassium acetate. Cyanidin-3-glucoside chloride (>98%) was obtained from Biopurify phytochemicals LTD (Chengdu, China).