In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at 40oC using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a 0.45 μm membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.
… acid(이하 3,4-diCQA, CAS Number: 14534-61-3, Purity: 98%), 3,5-
dicaffeoylquinic acid(이하 3,5-diCQA, CAS Number: 2450-53-5, Purity: 98%),
4,5-dicaffeoylquinic acid(이하 4,5-diCQA, CAS Number: 57378-72-0, Purity 98%）were purchased from Biopurify …