Effect of sample preparation on components and liver toxicity ofPolygonum multiflorum

G.P. Lv, L.Z. Meng, D.Q. Han,  H.Y. Li, J. Zhao , S.P. Li , 

Abstract

It was shown that different extracts had significant differences in the toxicity of Polygonum multiflorum. In this study, the effect of sample preparation on components and liver toxicity of different extracts from P. multiflorum were determined. Hepatoxic components were discovered based on biomembrane extraction. Comparative chemistry and toxicology between ethanol and water extracts were also performed. The results showed that ethanol extract had much stronger hepatotoxicity, the content of emodin-8-O-β-d-glucopyranoside, physcion-8-O-β-d-glucopyranoside, emodin and physcion was significantly higher in ethanol extract than in water extract, while the human hepatocytes extraction showed that 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucopyranoside,

Proanthocyanidin B1 and B2 were purchased from Chengdu Biopurify Phytochemicals Ltd (Chengdu, China).