Abstract
The objective of the work was to validate the high performance liquid chromatography for simultaneous determination of stability of madecassoside and asiaticoside in Centella asiatica (L.) Urb., Apiaceae, extract-loaded film forming polymeric dispersions. High performance liquid chromatography method was validated in five topics: linearity and range, limit of detection and limit of quantitation, specificity, precision, and accuracy. Results showed the method had a good linearity (R2 > 0.9990) in the range of 5–150 μg/ml and specific. The limit of detection and limit of quantitation of madecassoside were 81 and 245 ng/ml and asiaticoside were 21 and 64 ng/ml, respectively. The percent relative standard deviation of intraday and interday precision were less than 1 and 3%, respectively. The accuracy presented as percent recovery was 101.54–103.29% for madecassoside and 100.39–102.58% for asiaticoside. This validated high performance liquid chromatography method was used to determine the stability of the formulation containing Centella asiatica extract. Centella asiatica extract-loaded film forming polymeric dispersions used Eudragit® RS 30D and Eudragit® RL 30D as film former, glycerin as plasticizer, and absolute ethanol as solvent and penetration enhancer. Three formulations with different ratio of Eudragit® RS 30D and Eudragit® RL 30D were prepared and stored for 90 days at 4 °C, 25 °C, and 40 °C. Stability results showed that almost all of the formulations were unstable at 25 °C and 40 °C. Except, two of three formulations were stable at 4 °C. However, the formulation was further developed to improve the stability of madecassoside and asiaticoside in the formulation.
… Materials and methods. Chemicals and reagents. Madecassoside (purity 95.0%, HPLC) was purchased from Sigma–Aldrich Inc., Missouri, USA. Asiaticoside (purity 99.81%, HPLC) was purchased from Chengdu Biopurify Phytochemicals Ltd., Sichuan, China …
