Abstract
Oocyte in vitro maturation (IVM) plays a pivotal role in in vitro embryo production. However, the efficiency of IVM is still low and needs to be further improved. In the present study, we evaluated the beneficial effects of mogroside V, an extract derived from Siraitia grosvenorii, on oocyte IVM. Porcine cumulus–oocyte complexes were cultured in IVM medium supplemented or not supplemented with mogroside V for 40 h. We found that mogroside V supplementation increased the percentage of oocyte first polar body extrusion and improved subsequent blastocyst formation after parthenogenetic activation. Furthermore, mogroside V reduced the levels of reactive oxygen species (ROS) and increased the mRNA expression of oxidative stress-related genes (SOD, CAT and SIRT1). Moreover, mogroside V supplementation enhanced the mitochondrial content, mtDNA copy number, mitochondrial membrane potential (ΔΨm), ATP generation, and the relative mRNA expression of mitochondria-related genes (PGC-1α and TFAM). In summary, our findings demonstrate that mogroside V supplementation reduces intracellular ROS levels and enhances mitochondrial function to promote porcine oocyte IVM.
… incubator with humidified air. MV (Chengdu Biopurify Phytochemicals Ltd., Chengdu,China) was dissolved in water to a concentration of 100 mM, and stored at -20°C prior to use. Melatonin (Cat. #M5250) was dissolved in …
Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development
期刊名：Theriogenology
文献编号：
文献地址： https://www.sciencedirect.com/science/article/abs/pii/S0093691X19303929
发表日期：1 January 2019
文献编号：
文献地址： https://www.sciencedirect.com/science/article/abs/pii/S0093691X19303929
发表日期：1 January 2019
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