Abstract
Ginsenoside compound K (CK) is an ginseno side and has shown to have ameliorative properties in various diseases. However, the detailed in vivo metabolism of this compound has been rarely reported. In the present study, a method using liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry together with multiple data processing techniques, including extracted ion chromatogram, multiple mass defect filter, MSMS scanning was developed to screen and characterize the metabolites of CK in rat urine and feces. After oral administration of CK at a dose of 50 mg/kg, urine and feces were collected for a certain period of time and subjected to a series of pretreatment. A total of twelve metabolites were tentatively or conclusively identified, including eleven phase I metabolites and one phase II metabolite. Metabolism pathway of CK have been proposed, including prototype oxidation, deglycosylation, deglycosylation with sequential oxidation and dehydrogenation, deglycosylation with sequential glucuronidation. Relative quantitative analyses suggested that deglycosylation was the main metabolic pathway. The result could offer insights for better understanding the mechanism of its pharmacological activities.
… 2. Experimental 2.1. Chemicals and materials CK was purchased from Biopurify
Phytochemicals Ltd. (Chengdu, China) and confirmed by MS and NMR spectra.
The purity was above 98.0% as determined by a HPLC method …
