Abstract

We investigated the effect of polyphenols extracted from M. angusticepes Peck (PMP) on D-galactosamine/lipopolysaccharide (D-GalN/LPS) induced acute hepatic failure (AHF) in mice. Results indicated that administration of PMP relieved the leakage of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The level of serum ALT was reduced in groups of P-H (800?mg PMP/kg BW) and P-L (400?mg PMP/kg BW) by 44.6% and 31.8%, respectively. RT-PCR and Western blot analyses showed that NF-E2-related factor 2 (Nrf2), PPARα, PPARγ gene and protein were up-regulated after injection D-GalN/LPS. In contrast, PMP pretreatment noticeably prevented D-GalN/LPS-induced hepatocyte apoptosis and steatosis. It suggested that PMP attenuated D-GalN/LPS-induced AHF in mice, which was likely attributed to the co-activation of Nrf2, PPARα, PPARγ and alleviating the oxidative stress reaction, reducing the body inflammatory response and regulating the lipid metabolism.
Keywords
Morchella angusticepes Peck
Molecular mechanisms
Acute hepatic failure
PolyphenolsAbbreviationsAHF
Acute hepatic failure
Polyphenols
… Polyphenol standards (the orientin, protocatechuic acid, chlorogenic acid, etc.) were purchased from Chengdu Biopurify Phytochemicals (Chengdu, China). All other chemicals and reagents were purchased locally and were analytical grade. 2.2. Sample preparation …