Abstract

Three phenolic acids (gallic, 3,4-dihydroxybenzoic, and ellagic acids) and four hydrolyzable tannins (punicalin, punicalagins A and B, and corilagin) have been determined in pomegranate beverages and different parts of the fresh fruit and tree by liquid chromatography with diode array detection coupled to electrospray ionization and time-of-flight mass spectrometry (LC-DAD-ESI-ToFMS). For the fruit and tree analysis, the lyophilized sample was submitted to extraction in methanol for 2 h. Centrifuged and filtered beverages were diluted 1:10 with water for the quantification of corilagin and 3,4-dihydroxybenzoic acid and 1:100 for the rest of polyphenols. Calibration against external aqueous standards was used for quantification by liquid chromatography with
punicalin(PC, 98.6 % purity) by Chengdu Biopurify Phytochemicals Ltd. (Sichuan, China).