This study determined the content of chlorogenic acids (CA) and rutin during successive aqueous extraction of yerba mate for tererê and chimarr?o from four yerba mate types (smooth, native, traditional and coarse ground). Aqueous extracts were prepared aiming to simulate homemade procedure by partially (chimarr?o) or completely (tererê) immersing the herb into hot (chimarr?o) or cold (tererê) water (30 times consecutively). The content of CA and rutin in the aqueous extracts was compared to those in methanolic extracts (exhaustive extraction). Tererê aqueous extracts gave higher amounts of all phenolic compounds (2.5 to 6 times higher than chimarr?o). Among chimarr?o, course-ground aqueous extracts had on average 15% more PC (phenolic compounds). By comparing the content in aqueous and methanolic extracts, after 30 successive extractions, on average 14% of the total amount of CA in yerba mate leaves and 9% of rutin were transferred to the chimarr?o extracts, whereas tererê achieved between 40% and 100% of transference. Thus, this study shows that CA and rutin are continuously extracted during the preparation of aqueous extracts of chimarr?o and tererê, favoring a high intake of these antioxidant species by consumers.
... Water was purified using a Milli-Q? (Millipore) system. The phenolic compounds standards (5-caffeoylquinic acid, caffeic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, rutin and 4,5-dicaffeoylquinic acid) were obtained from Biopurify Phytochemicals Ltd. ...
