在线咨询
联系电话:
销售：
400-829-7929（7*24小时）
028-
82633860
028-
82633397
028-82633165
技术服务和产品定制：
028-82633987
在线服务:
沈帅 
文静
贺丹丹
文献信息
Polymethoxyflavones in peel of Citrus reticulata ‘Chachi’ and their biological activities
Citrus polymethoxyflavones (PMFs) have been of increasing interest due to their extensive biological activities. In the present study, a total of eight PMFs were isolated from the peel of Citrus reticulata ‘Chachi’ (CRC). They were individually identified as 5-hydroxy-6,7,8,4′-tetramethoxyflavone (1), 5,6,7,3′,4′-pentamethoxyflavanone (2), 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (3), 3,5,6,7,8,3′,4′- heptamethoxyflavone (4), 5,6,7,8,3′,4′-hexamethoxyflavone (5), 5,6,7,8,4′-pentamethoxyflavone (6), 6,7,8,3′,4′-pentamethoxyflavanone (7) and 5,6,7,3′,4′-pentamethoxyflavone (8) by nuclear magnetic resonance and mass spectroscopic analysis. 6,7,8,3′,4′-Pentamethoxyflavanone was isolated from the peel of CRC for the first time. The content of PMFs was firstly quantified in both the peel of CRC and PMF-rich extract by HPLC analysis. Furthermore, the biological activities of PMF compounds were investigated. 3,5,6,7,8,3′,4′-Heptamethoxyflavone demonstrated potent sterol regulatory element-binding proteins inhibition activity and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone exhibited strong antiproliferative activity against tumor cell lines. The isolated PMF compounds could also significantly inhibit NO production and the effect varied mainly depending on the number of methoxy groups.
... Louis, MO). Luciferase assay reagent and reporter lysis buffer were obtained from Promega (Madison, WI). Reference compounds of isosinensetin and 4',5,7,8-tetramethoxyflavone were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China). ...
