In vitro alpha glucosidase inhibition and free-radical scavenging activity of propolis from Thai stingless bees in mangosteen orchard

Boonyadist Vongsak , Sumet Kongkiatpaiboon, Sunan Jaisamut, Sasipawan Machana, Chamnan Pattarapanich

Abstract

The chemical component and biological activity of propolis depend on flora area of bee collection and bee species. In the study, the propolis from three stingless bee species,Lepidotrigona ventralis Smith, Lepidotrigona terminata Smith, and Tetragonula pagdeniSchwarz, was collected in the same region of mangosteen garden from Thailand. Total phenolic content, alpha glucosidase inhibitory effect, and free-radical scavenging activity using FRAP, ABTS, DPPH assays were determined. The most potent activity of propolis extract was investigated for bioactive compounds and their quantity. The ethanol extract ofT. pagdeni propolis had the highest total phenolic content

  Standard gamma-mangostin and alpha-mangostin purity more than 98% were purchased from Chengdu Biopurify Phytochemicals Ltd, Sichuan,China.