1-Deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) is the first committed enzyme in the MEP terpenoid biosynthetic pathway and also a validated antimicrobial target. Green tea which is rich in polyphenolic components such as the catechins, possesses a plenty of pharmacological activities, in particular an antibacterial effect. To uncover the antibacterial mechanism of green tea and to seek new DXR inhibitors from natural sources, the DXR inhibitory activity of green tea and its main antimicrobial catechins were investigated in this study. The results show that the raw extract of green tea and its ethyl acetate fraction are able to suppress DXR activity explicitly. Further determination of the DXR inhibitory capacity of eight catechin compounds demonstrates that the most active compound is gallocatechin gallate that is able to inhibit around 50% activity of DXR at 25 μM. Based on these data, the primary structure-activity relationship of the catechins against DXR is discussed. This study would be very helpful to elucidate the antimicrobial mechanism of green tea and the catechins and also would be very useful to direct the rational utilization of them as food additives.
... It was delivered in ice and then stored at ? 80 °C before use. The catechins were purchased from Chengdu Biopurify Phytochemicals, Ltd. (Chengdu, China), and the stock solutions of them (10 mg/mL) were prepared in 40% aqueous DMSO just before use. ...
Inhibition of green tea and the catechins against 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway
期刊名：Fitoterapia
文献编号：
文献地址： http://www.sciencedirect.com/science/article/pii/S0367326X16301666
发表日期：September 2016
文献编号：
文献地址： http://www.sciencedirect.com/science/article/pii/S0367326X16301666
发表日期：September 2016
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