The effects of fibrous root of Rhizoma Coptidis (FRC) and its main alkaloids on non-specific immunity and disease resistance of common carp were investigated. The fish were randomly assigned to seven groups: normal control group (NC), groups treated with 12.5 g/kg FRC (FRC-L), 25 g/kg FRC (FRC-M), 50 g/kg FRC (FRC-H), 0.78 g/kg total alkaloids (TA), 0.78 g/kg berberine (BBR), and 0.78 g/kg coptisine (Cop), respectively. Results showed that the activities of myeloperoxidase, lysozyme and respiratory burst were significantly elevated after treated with FRC-M, FRC-H, TA, BBR and Cop, and the complement C3 level and phagocytic activity were significantly increased in FRC-M, TA and BBR treated groups compared with NC group. The real-time PCR analysis indicated that FRC, TA, BBR and Cop could up-regulate the mRNA expression of IL-1β, TNF-α, lysozyme-c and C3, but down-regulate that of IL-10 in the head kidney of common carp. Besides, FRC-M, FRC-H, TA, BBR and Cop significantly enhanced the survival rate of common carp infected with Aeromonas hydrophila, when compared to NC group. It was concluded that the FRC could enhance the non-specific immunity and disease resistance of common carp and the main alkaloids might contribute to these effects.
... resistance of common carp were investigated. 2. Materials and methods. 2.1. Chemicals. The standard of berberine (BBR) was purchased from Chengdu Biopurify Phytochmicals Ltd. (Chengdu China). Total alkaloids (TA) were the ...
Effects of dietary supplementations with the fibrous root of Rhizoma Coptidis and its main alkaloids on non-specific immunity and disease resistance of common carp
期刊名：Veterinary Immunology and Immunopathology
文献编号：
文献地址： http://www.sciencedirect.com/science/article/pii/S0165242716300393
发表日期：May 2016
文献编号：
文献地址： http://www.sciencedirect.com/science/article/pii/S0165242716300393
发表日期：May 2016
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