A method for extracting chlorogenic acid (CA) avoiding the use of expensive organic solvents was developed based on studies of the processing of green coffee beans with subcritical water. The content of chlorogenic acid in the obtained extracts was determined by high-performance liquid chromatography (HPLC). The method was shown to be more effective than the known ethanol extraction procedure.
... Quantitative determination of CA in the extracts was performed by absolute calibration. The
standard was the isomer of 3 O caffeoylquinic acid (99.1%, Chengdu Biopurify Phytochemicals,
CAS no. 327 97 9, Catalog no. C07007). RESULTS AND DISCUSSION ...
