Ginsenoside Rg1 suppresses early stage of adipocyte development via activation of C/EBP homologous protein-10 in 3T3-L1 and attenuates fat accumulation in high fat diet-induced obese zebrafish
Eun-Jeong Koh, Kui-Jin Kim, Jia Choi, Hui Jeon Jeon, Min-Jung Seo, Boo-Yong Lee
Abstract
Background
Ginsenoside Rg1 is a class of steroid glycoside and triterpene saponin in Panax ginseng. Many studies suggest that Rg1 suppresses adipocyte differentiation in 3T3-L1. However, the detail molecular mechanism of Rg1 on adipogenesis in 3T3-L1 is still not fully understood.
Methods
3T3-L1 preadipocyte was used to evaluate the effect of Rg1 on adipocyte development in the differentiation in a stage-dependent manner in vitro. Oil Red O staining and Nile red staining were conducted to measure intracellular lipid accumulation and superoxide production, respectively. We analyzed the protein expression using Western blot in vitro. The zebrafish model was used to investigate whether Rg1 suppresses the early stage of fat accumulation in vivo.
Materials 3 Ginsenoside Rg1 (≥ 95% purity) was purchased from ChengDu Biopurify 4 Phytochemicals Ltd (Biopurify, ChengDu, China).
