This study sought to identify a new strain of microorganism from a natural source, nuruk, for use in the production of platycodin D (PD) from the roots of Platycodon grandiflorum (PG). A non-Saccharomyces yeast capable of producing PD was isolated from nuruk and identified as Cyberlindnera fabianii. The enzyme responsible for PD production was traced with partially purified crude enzyme extracts (CEE) from yeast C. fabianii 48. The best enzyme fraction responsible for production of PD was the >100?kDa CEE. In addition, the bioconversion pathway for production of PD by the >100?kDa CEE with PD, platycodin D2 (PD2) and platycodin D3 (PD3) substrates was investigated. The >100?kDa CEE was able to degrade PD3 to PD and had no effect on PD and PD2.
Platycodin D2 (PD2), PD3 and PD standards were purchased from
Biopurify phytochemicals Ltd (Chengdu, Sichuan, China).
