Simultaneous Analysis of the Chemical Compounds in Ojeok-san and Its Antioxidative Activity

Abstract

Ojeok-san has been commonly used to treat low back pain disease. We performed the experiments on simultaneous analysis of 11 compounds and antioxidant effects of Ojeok-san. A HPLC method was established for simultaneous analysis of 11 compounds. The detection wavelengths were set at 230, 254, 280, 320, and 330 nm. The detected 11 compounds from Ojeok-san water extract showed good linearity(r 2 ≥0.9997). Limit of detection(LOD) and limit of quantification(LOQ) were 0.04-0.87 μg/mL and 0.13-2.63 μg/mL, respectively. The antioxidant effects of Ojeok-san water extract were investigated by DPPH and ABTS assays. Ojeok-san water extract significantly increased the DPPH and ABTS radical scavenging effects in a dose-dependent manner. The RC 50 value of Ojeok-san on DPPH radical was 284.71 μg/mL and that of ABTS radical was 96.16 μg/mL.

 Hesperidin,neohesperidin(purity>98%) Biopurify Phytochemicals Ltd.(Chengdu,