Simultaneous quantification of polyherbal formulations containing Rhodiola rosea L. and Eleutherococcus senticosusMaxim. using rapid resolution liquid chromatography (RRLC)

Yuan-Chun Ma, Xiao-Qiang Wang, FeiFei Hou, Jie Ma , Mai Luo,Shane Lu, Peter Jin, Alice Chen, Iris Xu, Asmita V. Patel, Derek Gorecki

Abstract

An RRLC method capable of simultaneous identification and rapid quantification of six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin, rosiridin) inRhodiola rosea L. and two active compounds (eleutheroside B and eleutheroside E) inEleutherococcus senticosus Maxim. was developed. The chromatographic analyses were performed on a reversed phase Phenomenex C18 (2)-HST column at 40 °C with a neutral mobile phase (purified water and acetonitrile) gradient system at a flow rate of 1.0 ml/min and UV detection at 205 and 220 nm simultaneously. Baseline separation of eight active compounds was achieved within 8 min. This developed method provides good linearity (R > 0.9997), precision (RSD < 1.99%) and recovery of the bioactive compounds. The RRLC method developed is capable of controlling the quality of R. rosea and E. senticosusraw herbs, commercial extracts, as well as polyherbal formulations containing R. rosea andE. senticosus as ingredients. This RRLC method is accurate and sensitive;

 eleutheroside B (purity: 99.6%),eleutheroside E (purity: 99.1%) and rosiridin were purchased from Biopurify (Chengdu, China