Abstract

Objectives

Gwakhyangjeonggi-san (GJS) which consists of 13 herbal medicines has been used to treat gastrointestinal disorders caused by common cold. This study was performed to compare GJS decoctions produced using different pressure levels for various extraction times.

Methods

Decoctions were prepared by the pressure levels of 0 kgf/cm 2 (non-pressurized) or 1 kgf/cm 2 (pressurized) for 30–180 min. The extraction yield, total soluble solid content (TSSC), and hydrogen ion concentration (pH) were measured, and the contents of the nine marker compounds were determined using high performance liquid chromatography.

Results

The higher pressure and longer extraction time significantly increased TSSC value, while decreased the pH value. However, only extraction time affected the extraction yield of pressurized decoction. Variation of the amounts of chemical compounds was shown in pressurized and non-pressurized decoctions during extraction time. The result of regression analysis showed that pressure and extraction time can influence to extraction yield, TSSC, pH, and the content of chemical compounds.

Apigetrin, oxypeucedanin hydrate, and byakangelicin were supplied from Chengdu Biopurify Phytochemicals Ltd (Chengdu, China),