Ethanol extract of Alismatis Rhizoma reduces acute lung inflammation by suppressing NF-κB and activating Nrf2

Chang Woo Han, Min Jung Kwun, Kyun Ha Kim, Jun-Yong Choi, Sei-Ryang Oh,Kyung-Seop Ahn, Jang Hoon Lee , Myungsoo Joo, 

Abstract

Ethnopharmacological relevance

The tuber of Alisma orientale Juzepzuk, a medicinal herb that has been used for the treatment of various disorders in Korea, has an anti-inflammatory effect. Here, we investigated a possible underlying mechanism and a protective effect on acute lung injury (ALI).

Materials and methods

Alisma orientale tuber was extracted in 80% ethanol and dried. The powder of the ethanol extract of Alisma orientale tuber (EEAO) was dissolved in PBS. The effect of EEAO on NF-κB and Nrf2 activities was analyzed with RAW 264.7 cells. The effect of EEAO on lung inflammation was determined by histologic and molecular biological analyses of the lung tissue of C57BL/6 mice that were gavaged once a day with 0.3 or 1.2 g/kg of EEAO for 14 days, prior to an intranasal administration of LPS (0.01 g/kg) for inducing ALI.

Alisol acetate B for an index component (Lee et al., 2004) was purchased from Biopurify Phytochemical Co. Ltd (Chengdu, Sichuan, China).