Abstract

A simple and specific method for the simultaneous determination of eleutherosides B and E in powdered rhizomes of Eleutherococcus senticosus extract and in solid and liquid dietary supplements was developed and validated. E. senticosus extracts, often mixed with other plants or herbal extracts, are widely used in food supplements because of the tonic and adaptogenic activities referred to the eleutherosides B and E. In this study, samples were analyzed by a liquid chromatography-electrospray tandem mass spectrometry (LC–ESI-MS/MS) method operated in single reaction monitoring (SRM). Validation was carried out in terms of limit of detection (LOD), limit of quantitation (LOQ), linearity, precision and trueness. LOD and LOQ values were fixed at 3 μg L?1and 10 μg L?1, respectively, whereas linearity was established within 10–1,000 μg L?1 range for both compounds. Good precision was obtained for both eleutherosides in terms of intra-day precision (RSD % lower than 4 %) and inter-day precision (RSD % lower than 6 %). Good percentage recoveries were obtained for both eleutherosides (91.5–103.6 %). Finally, the developed method was 

Eleutheroside B (4-(3-hydroxy-1-propenyl)-2,6-dime- thoxyphenyl b-D-glucopiranoside [99.6 %)
and eleuthero- side E (syringaresinol di-Ob-D-glucopyranoside[99.1 %) as reference standards
were purchased from Biopurify Phytochemical (Chengdu, Sichuan, China)