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Hyekyung Ha, Hoyoung Lee, Chang-Seob Seo, Hye-Sun Lim, Mee-Young Lee, Jun Kyoung Lee, and Hyeunkyoo Shin
Aims.Artemisia iwayomogi (AI) has been used for fever reduction, diuresis, and hepatoprotection in Korea. The present study was performed to evaluate the anti-inflammatory and antiatopic dermatitis effects of AI using bothin vitro and in vivo systems. Methods. The compositions in AI were analyzed by HPLC. To determine the anti-inflammatory effects of AI, the production of nitric oxide (NO) was measured in lipopolysaccharide treated RAW264.7 cells. Histamine levels were assayed to evaluate the antiallergic effects on MC/9 cells stimulated with phorbol-12 myristate 13-acetate and A23187. Finally, AI (10 mg/mouse/day) was topically applied onto the backs and ears of Dermatophagoides farinae-sensitized Nc/Nga mice for four weeks. Results. Isochlorogenic acid A (20.63~0.26mg/g), chlorogenic acid (9.04 ~0.08mg/g), and scopoletin (8.23 ~ 0.01mg/g)
Mogrosides IV and V were purchased from Chengdu Biopurify Photochemicals Co. (Chengdu, China)