VNU Journal of Science: Medical and Pharmaceutical Sciences, Vol. 40, No. 4 (2024) 1-10
1
Original Article
Development of an HPLC Method
for Simultaneous Quantitation of Hyperin, Quercetin
and Kaempferol in the Semen cuscutae
Do Thi Dinh3, Nguyen Thi Minh Diep1, Tran Thi Van Anh1, Ha Thanh Hoa1,
Dao Viet Hung1, Ngo Thi Xuan Thinh1, Nguyen Thanh Hai2, Pham Quoc Tuan1,*
1Phu Tho College of Medicine and Pharmacy, 2201 Hung Vuong, Gia Cam, Viet Tri, Phu Tho, Vietnam
2VNU University of Medicine and Pharmacy, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam
3Hai Duong Central College of Pharmacy, 324 Nguyen Luong Bang, Thanh Binh, Hai Duong, Vietnam
Received 15 October 2024
Revised 22 November 2024; Accepted 25 November 2024
Abstract: In this study, a method for the simultaneous quantification of hyperin, quercetin, and
kaempferol in samples of Semen cuscutae was developed using high-performance liquid
chromatography (HPLC). The chosen chromatographic conditions were a mobile phase of methanol
– 0.1% formic acid in water, running in gradient mode, with a flow rate of 0.9 ml/min, and detection
wavelengths of 354, 365, and 369 nm for hyperin, kaempferol, and quercetin, respectively. The
results of the quantitative analysis of 5 samples of Semen cuscutae purchased from Phu Tho province
showed hyperin content ranging from 0.950 to 1.218 mg/g; quercetin from 0.077 to 0.118 mg/g; and
kaempferol from 1.051 to 1.190 mg/g based on the dry weight of the medicinal material. This
research may be suitable for the quality control of Semen cuscutae in the market
