Acanthopanax senticosus belongs to Araliaceae family and is traditionally used as a tonic. The18
roots and stems are mainly used as a treatment for hypodynamia, rheumatism, and hypertension,19
but their frequent use may lead to its extinction. However, comprehensive and simultaneous20
analysis of the remaining parts were still limited, and needs to reorganize for standardization21
of functional foods. In this study, 50 phenolic compounds and 82 triterpenoid saponins from22
the shoots, leaves, fruits, and stems of A. senticosus were characterized using UPLC-QTOF-23
MS and UPLC-QTRAP-MS/MS. Among them, 57 new compounds were detected as the cis24
and malonyl-bound phenolic acids and were structural isomers of Acanthopanax flavonoids25
and saponins. All compounds were absolutely/relatively quantified, and shoots had the highest26
content. Antioxidant and α-glucosidase inhibitory activity were performed, followed by27
evaluation of structure-activity relationships. Both activities of all tested extracts and saponins28
showed remarkable efficacy and affected by the C-23 hydroxylation, the C-20(29) double bond,29
and the presence of rhamnose. These detailed profiling can be used as fundamental data for30
increasing the utilization of A. senticosus and developing them into functional foods.