Purpose: As the demand for natural cosmetics increases, properly managing
production and quality control should be prioritized. Chlorogenic acid (CGA),
epicatechin gallate (ECG), and rosmarinic acid (RMA) were selected to validate the
cosmetic composite LOL, a mixture of 50% EtOH extract of
Ligularia stenocephala
(LS),
Orostachys japonica (OJ), and
Lavandula angustifolia (LA). Methods: High-
performance liquid chromatography with a diode array detector (HPLC-DAD) was
used to simultaneously analyze the three LOL compounds from LOL (
Ligularia
stenocephala,
Orostachys japonica,
Lavandula angustifolia). Linearity, accuracy, and
precision analyses were performed using HPLC-DAD, and the anti-oxidative effects
were evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging
assay. Results: All three extracts showed a high DPPH radical-scavenging effect, and
the detected marker compounds showed significant linearity (R2≥0.9997). The limit
of detection (LOD) of CGA, ECG, and RMA was 0.83 μg/mL, 0.33 μg/mL, and 0.20
μg/mL, respectively. Meanwhile, the limit of quantification (LOQ) of CGA, ECG, and
RMA was 2.51 μg/mL, 0.99 μg/mL, and 0.61 μg/mL, respectively. Method validation
showed acceptable precision (intra-and inter-day precision, CGA, ECG, and RMA, 1.77,
1.42, 0.80% and 1.09, 0.72, 1.42%, respectively) and recovery (CGA, ECG, and
RMA, 102.00%–117.78%, 94.40%-108.06%, and 93.79%–107.05%). Conclusion:
The contents of the three markers were 13.99 μg/mL CGA, 8.28 μg/mL ECG, and
8.40 μg/mL of RMA, respectively. This study also suggests that the developed
method can validate the LOL cosmetic composition quality control process.