Objective – to verify the antimicrobial action of Oleuropein alone and together as Sodium Hypochlorite 1%. Methods – The resistance to sanitizers was evaluated by the Minimum Inhibitory Concentration (CIM) for SA (strain ATCC 25923) Laborclin® Brasil, using the dilution method in broth and incubated at 37ºC / 24h for comparison of tubes. For the disc agar diffusion method (DDA), after correction of the bacteria in 108 CFU (0.5 McFarland scale), they were plated using the standardized cotton swab technique to spread the suspension on the Petri dish containing TSA (Triptych Soy Agar, Sigma-Aldrich). 6mm discs of filter paper were placed, soaked in sanitizers and incubated in an oven at 37ºC / 24h, followed by the reading of the inhibition halos. Results – Turbidity and the tip of Ole inhibition halo more effective against Staphylococcus aureus cells in relation to HS 1%. This is due to the phosphatidylglycerol present in the derivatives of Oliveira. Conclusion – OLE has a sanitizing effect against S. aureus, however further studies are needed. HS should not be diluted to clean hospital surfaces so as not to lose its antibacterial effect.